Department School of Medicine, School of Medicine Position Assistant Professor
|Article types||Original article|
|Peer review||Peer reviewed|
|Title||In situ hybridization analysis of the expression of futsch, tau, and MESK2 homologues in the brain of the European honeybee (Apis mellifera L.).|
|Journal||Formal name：PLoS ONE|
|Domestic / Foregin||Foregin|
|Volume, Issue, Page||5(2),e9213頁|
|Author and coauthor||Kaneko Kumi†, Hori Sayaka, Morimoto Mai, Nakaoka Takayoshi , Paul Rajib Kumar, Fujiyuki Tomoko, Shirai Ken-ichi, Wakamoto Akiko, Tsuboko Satomi, Takeuchi Hideaki, Kubo Takeo*|
Expression analysis of candidate genes using in situ hybridization revealed two genes expressed in a neural cell-type preferential manner in the OLs. One is a homologue of Drosophila futsch, which encodes a microtubule-associated protein and is preferentially expressed in the monopolar cells in the lamina of the OLs. The gene for another microtubule-associated protein, tau, which functionally overlaps with futsch, was also preferentially expressed in the monopolar cells, strongly suggesting the functional importance of these two microtubule-associated proteins in monopolar cells. The other gene encoded a homologue of Misexpression Suppressor of Dominant-negative Kinase Suppressor of Ras 2 (MESK2), which might activate Ras/MAPK-signaling in Drosophila. MESK2 was expressed preferentially in a subclass of neurons located in the ventral region between the lamina and medulla neuropil in the OLs, suggesting that this subclass is a novel OL neuron type characterized by MESK2-expression. These three genes exhibited similar expression patterns in the worker, drone, and queen brains, suggesting that they function similarly irrespective of the honeybee sex or caste.
Here we identified genes that are expressed in a monopolar cell (Amfutsch and Amtau) or ventral medulla-preferential manner (AmMESK2) in insect OLs. These genes may aid in visualizing neurites of monopolar cells and ventral medulla cells, as well as in analyzing the function of these neurons.