Dejima Katsufumi    Department   School of Medicine, School of Medicine    Position   Assistant Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Distinct pathways for export of silencing RNA in Caenorhabditis elegans systemic RNAi.
Journal	Formal name：iScience Abbreviation：iScience ISSN code：25890042/25890042
Domestic / Foregin	Foregin
Volume, Issue, Page	26(10),pp.108067
Author and coauthor	YOSHIDA Keita†, SUEHIRO Yuji, DEJIMA Katsufumi, YOSHINA Sawako, MITANI Shohei*
Publication date	2023/10
Summary	Dietary supplied double-stranded RNA (dsRNA) can trigger RNA interference (RNAi) systemically in some animals, including the nematode Caenorhabditis elegans. Although this phenomenon has been utilized as a major tool for gene silencing in C. elegans, how cells spread the silencing RNA throughout the organism is largely unknown. Here, we identify two novel systemic RNAi-related factors, REXD-1 and TBC-3, and show that these two factors together with SID-5 act redundantly to promote systemic spreading of dsRNA. Animals that are defective in all REXD-1, TBC-3, and SID-5 functions show strong deficiency in export of dsRNA from intestinal cells, whereas cellular uptake and processing of dsRNA and general secretion events other than dsRNA secretion are still functional in the triple mutant animals. Our findings reveal pathways that specifically regulate the export of dsRNA in parallel, implying the importance of spreading RNA molecules for intercellular communication in organisms.
DOI	10.1016/j.isci.2023.108067
PMID	37854694