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Dejima Katsufumi
Department School of Medicine, School of Medicine Position Assistant Professor |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | An Aneuploidy-Free and Structurally Defined Balancer Chromosome Toolkit for Caenorhabditis elegans. |
| Journal | Formal name：Cell reports Abbreviation：Cell Rep ISSN code：(2211-1247) |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 22(1),pp.232-241 |
| Author and coauthor | Dejima Katsufumi†, Hori Sayaka†, Iwata Satoru†, Suehiro Yuji, Yoshina Sawako, Motohashi Tomoko, Mitani Shohei* |
| Authorship | Lead author |
| Publication date | 2018/01 |
| Summary | Balancer chromosomes are critical tools for genetic research. In C. elegans, reciprocal translocations that lead to aneuploidy have been widely used to maintain lethal and sterile mutations in stable stocks. Here, we generated a set of aneuploidy-free and structurally defined crossover suppressors that contain two overlapping inversions using the CRISPR/Cas9 system. The toolkit includes 13 crossover suppressors and covers approximately 63% of all C. elegans coding genes. Together with the classical intrachromosomal crossover suppressors, the system now covers 89% of the coding genes. We also labeled the created balancers with fluorescent and phenotypic markers. We show that the crossover suppressors are better for embryonic analysis compared with translocational balancers. Additionally, we demonstrate an efficient method to generate lethal alleles by targeting essential genes on a chromosome balanced with a crossover suppressor. The toolkit will allow more efficient experiments in which lethal and sterile mutants can be analyzed. |
| DOI | 10.1016/j.celrep.2017.12.024 |
| PMID | 29298424 |