DEJIMA Katsufumi    Department   School of Medicine, School of Medicine    Position   Assistant Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Chondroitin 4-O-Sulfotransferase Is Indispensable for Sulfation of Chondroitin and Plays an Important Role in Maintaining Normal Life Span and Oxidative Stress Responses in Nematodes.
Journal	Formal name：The Journal of biological chemistry Abbreviation：J Biol Chem ISSN code：(1083-351X)0021-9258(Linking)
Domestic / Foregin	Foregin
Volume, Issue, Page	291(44),pp.23294-23304
Author and coauthor	Izumikawa Tomomi, Dejima Katsufumi, Watamoto Yukiko, Nomura Kazuko H, Kanaki Nanako, Rikitake Marika, Tou Mai, Murata Daisuke, Yanagita Eri, Kano Ai, Mitani Shohei, Nomura Kazuya, Kitagawa Hiroshi
Authorship	2nd author
Publication date	2016/10
Summary	Chondroitin sulfate (CS)/chondroitin (Chn) chains are indispensable for embryonic cell division and cytokinesis in the early developmental stages in Caenorhabditis elegans and mice, whereas heparan sulfate (HS) is essential for axon guidance during nervous system development. These data indicate that the fundamental functions of CS and HS are conserved from worms to mammals and that the function of CS/Chn differs from that of HS. Although previous studies have shown that C. elegans produces HS and non-sulfated Chn, whether the organism produces CS remains unclear. Here, we demonstrate that C. elegans produces a small amount of 4-O-sulfated Chn and report the identification of C41C4.1, an orthologue of the human chondroitin 4-O-sulfotransferase gene. Loss of C41C4.1 in C. elegans resulted in a decline in 4-O-sulfation of CS and an increase in the number of sulfated units in HS. C41C4.1 deletion mutants exhibited reduced survival rates after synchronization with sodium hypochlorite. Collectively, these results show for the first time that CS glycans are present in C. elegans and that the Chn 4-O-sulfotransferase responsible for the sulfation plays an important role in protecting nematodes from oxidative stress.
DOI	10.1074/jbc.M116.757328
PMID	27645998