デジマ カツフミ   Dejima Katsufumi
  出嶋 克史
   所属   医学部 医学科
   職種   助教
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Chondroitin 4-O-Sulfotransferase Is Indispensable for Sulfation of Chondroitin and Plays an Important Role in Maintaining Normal Life Span and Oxidative Stress Responses in Nematodes.
掲載誌名 正式名:The Journal of biological chemistry
略  称:J Biol Chem
ISSNコード:(1083-351X)0021-9258(Linking)
掲載区分国外
巻・号・頁 291(44),23294-23304頁
著者・共著者 Izumikawa Tomomi, Dejima Katsufumi, Watamoto Yukiko, Nomura Kazuko H, Kanaki Nanako, Rikitake Marika, Tou Mai, Murata Daisuke, Yanagita Eri, Kano Ai, Mitani Shohei, Nomura Kazuya, Kitagawa Hiroshi
発行年月 2016/10
概要 Chondroitin sulfate (CS)/chondroitin (Chn) chains are indispensable for embryonic cell division and cytokinesis in the early developmental stages in Caenorhabditis elegans and mice, whereas heparan sulfate (HS) is essential for axon guidance during nervous system development. These data indicate that the fundamental functions of CS and HS are conserved from worms to mammals and that the function of CS/Chn differs from that of HS. Although previous studies have shown that C. elegans produces HS and non-sulfated Chn, whether the organism produces CS remains unclear. Here, we demonstrate that C. elegans produces a small amount of 4-O-sulfated Chn and report the identification of C41C4.1, an orthologue of the human chondroitin 4-O-sulfotransferase gene. Loss of C41C4.1 in C. elegans resulted in a decline in 4-O-sulfation of CS and an increase in the number of sulfated units in HS. C41C4.1 deletion mutants exhibited reduced survival rates after synchronization with sodium hypochlorite. Collectively, these results show for the first time that CS glycans are present in C. elegans and that the Chn 4-O-sulfotransferase responsible for the sulfation plays an important role in protecting nematodes from oxidative stress.
DOI 10.1074/jbc.M116.757328
PMID 27645998