デジマ カツフミ   Dejima Katsufumi
  出嶋 克史
   所属   医学部 医学科
   職種   助教
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Drosophila heparan sulfate 6-O endosulfatase regulates Wingless morphogen gradient formation.
掲載誌名 正式名:Developmental biology
略  称:Dev Biol
ISSNコード:1095-564X(Electronic)0012-1606(Linking)
巻・号・頁 345(2),204-14頁
著者・共著者 Kleinschmit Adam, Koyama Takashi, Dejima Katsufumi, Hayashi Yoshiki, Kamimura Keisuke, Nakato Hiroshi
発行年月 2010/09
概要 Heparan sulfate proteoglycans (HSPGs) play critical roles in the distribution and signaling of growth factors, but the molecular mechanisms regulating HSPG function are poorly understood. Here, we characterized Sulf1, which is a Drosophila member of the HS 6-O endosulfatase class of HS modifying enzymes. Our genetic and biochemical analyses show that Sulf1 acts as a novel regulator of the Wg morphogen gradient by modulating the sulfation status of HS on the cell surface in the developing wing. Sulf1 affects gradient formation by influencing the stability and distribution of Wg. We also demonstrate that expression of Sulf1 is induced by Wg signaling itself. Thus, Sulf1 participates in a feedback loop, potentially stabilizing the shape of the Wg gradient. Our study shows that the modification of HS fine structure provides a novel mechanism for the regulation of morphogen gradients.
DOI 10.1016/j.ydbio.2010.07.006
文献番号 20637191