デジマ カツフミ   Dejima Katsufumi
  出嶋 克史
   所属   医学部 医学科
   職種   助教
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 GPI-anchor synthesis is indispensable for the germline development of the nematode Caenorhabditis elegans.
掲載誌名 正式名:Molecular biology of the cell
略  称:Mol Biol Cell
ISSNコード:1939-4586(Electronic)1059-1524(Linking)
巻・号・頁 23(6),982-95頁
著者・共著者 Murata Daisuke, Nomura Kazuko H, Dejima Katsufumi, Mizuguchi Souhei, Kawasaki Nana, Matsuishi-Nakajima Yukari, Ito Satsuki, Gengyo-Ando Keiko, Kage-Nakadai Eriko, Mitani Shohei, Nomura Kazuya
発行年月 2012/03
概要 Glycosylphosphatidylinositol (GPI)-anchor attachment is one of the most common posttranslational protein modifications. Using the nematode Caenorhabditis elegans, we determined that GPI-anchored proteins are present in germline cells and distal tip cells, which are essential for the maintenance of the germline stem cell niche. We identified 24 C. elegans genes involved in GPI-anchor synthesis. Inhibition of various steps of GPI-anchor synthesis by RNA interference or gene knockout resulted in abnormal development of oocytes and early embryos, and both lethal and sterile phenotypes were observed. The piga-1 gene (orthologue of human PIGA) codes for the catalytic subunit of the phosphatidylinositol N-acetylglucosaminyltransferase complex, which catalyzes the first step of GPI-anchor synthesis. We isolated piga-1-knockout worms and found that GPI-anchor synthesis is indispensable for the maintenance of mitotic germline cell number. The knockout worms displayed 100% lethality, with decreased mitotic germline cells and abnormal eggshell formation. Using cell-specific rescue of the null allele, we showed that expression of piga-1 in somatic gonads and/or in germline is sufficient for normal embryonic development and the maintenance of the germline mitotic cells. These results clearly demonstrate that GPI-anchor synthesis is indispensable for germline formation and for normal development of oocytes and eggs.
DOI 10.1091/mbc.E10-10-0855
文献番号 22298425