Dejima Katsufumi
   Department   School of Medicine, School of Medicine
   Position   Assistant Professor
Article types Original article
Language English
Peer review Peer reviewed
Title RNAi screening of human glycogene orthologs in the nematode Caenorhabditis elegans and the construction of the C. elegans glycogene database.
Journal Formal name:Glycobiology
Abbreviation:Glycobiology
ISSN code:1460-2423(Electronic)0959-6658(Linking)
Volume, Issue, Page 25(1),8-20頁
Author and coauthor Akiyoshi Sayaka, Nomura Kazuko H, Dejima Katsufumi, Murata Daisuke, Matsuda Ayako, Kanaki Nanako, Takaki Tetsuro, Mihara Hiroyuki, Nagaishi Takayuki, Furukawa Shuhei, Ando Keiko-Gengyo, Yoshina Sawako, Mitani Shohei, Togayachi Akira, Suzuki Yoshinori, Shikanai Toshihide, Narimatsu Hisashi, Nomura Kazuya
Publication date 2015/01
Summary UNLABELLED:In this study, we selected 181 nematode glycogenes that are orthologous toUNLABELLED:human glycogenes and examined their RNAi phenotypes. The results are deposited in the Caenorhabditis elegans Glycogene Database (CGGDB) at AIST, Tsukuba, Japan. The most prominent RNAi phenotypes observed are disruptions of cell cycle progression in germline mitosis/meiosis and in early embryonic cell mitosis. Along with the previously reported roles of chondroitin proteoglycans, glycosphingolipids and GPI-anchored proteins in cell cycle progression, we show for the first time that the inhibition of the functions of N-glycan synthesis genes (cytoplasmic alg genes) resulted in abnormal germline formation, ER stress and small body size phenotypes. The results provide additional information on the roles of glycoconjugates in the cell cycle progression mechanisms of germline and embryonic cells.
DOI 10.1093/glycob/cwu080
Document No. 25091817