デジマ カツフミ   Dejima Katsufumi
  出嶋 克史
   所属   医学部 医学科
   職種   助教
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Syndecan defines precise spindle orientation by modulating Wnt signaling in C. elegans.
掲載誌名 正式名:Development (Cambridge, England)
略  称:Development
ISSNコード:1477-9129(Electronic)0950-1991(Linking)
巻・号・頁 141(22),4354-65頁
著者・共著者 Dejima Katsufumi, Kang Sukryool, Mitani Shohei, Cosman Pamela C, Chisholm Andrew D
発行年月 2014/11
概要 Wnt signals orient mitotic spindles in development, but it remains unclear how Wnt signaling is spatially controlled to achieve precise spindle orientation. Here, we show that C. elegans syndecan (SDN-1) is required for precise orientation of a mitotic spindle in response to a Wnt cue. We find that SDN-1 is the predominant heparan sulfate (HS) proteoglycan in the early C. elegans embryo, and that loss of HS biosynthesis or of the SDN-1 core protein results in misorientation of the spindle of the ABar blastomere. The ABar and EMS spindles both reorient in response to Wnt signals, but only ABar spindle reorientation is dependent on a new cell contact and on HS and SDN-1. SDN-1 transiently accumulates on theABar surface as it contacts C, and is required for local concentration of Dishevelled (MIG-5) in the ABar cortex adjacent to C. These findings establish a new role for syndecan in Wnt-dependent spindle orientation.
DOI 10.1242/dev.113266
文献番号 25344071