Homma Jun
Department Research Institutes and Facilities, Research Institutes and Facilities Position Assistant Professor |
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Article types | Original article |
Language | English |
Peer review | Peer reviewed |
Title | Mesenchymal stem cell sheets exert anti-stenotic effects in a rat arterial injury model. |
Journal | Formal name:Tissue engineering. Part A. Abbreviation:Tissue Eng Part A ISSN code:19373341/1937335X |
Domestic / Foregin | Foregin |
Volume, Issue, Page | 24(19-20),pp.1545-1553 |
Author and coauthor | HOMMA Jun†, SEKINE Hidekazu*, MATSUURA Katsuhisa, SHIMIZU Tatsuya |
Authorship | Lead author |
Publication date | 2018/10 |
Summary | Restenosis after catheter or surgical intervention substantially affects the prognosis of arterial occlusive disease. Mesenchymal stem cells (MSCs) may have anti-stenotic effects on injured arteries. MSC transplantation from the adventitial side of an artery is safer than endovascular transplantation but has not been extensively examined. In this study, a rat model of femoral artery injury was used to compare the anti-stenotic effects of transplanted cell sheets and transplanted cell suspensions. Rat adipose-derived stem cells (ASCs) were used as the source of MSCs. For both cell sheets and suspensions, 6×106 MSCs were transplanted on the day of arterial injury. MSC sheets attenuated neointimal hyperplasia more than MSC suspensions (intima-to-media ratio in haematoxylin/eosin-stained sections: 0.55±0.13 vs. 1.14±0.12; P<0.05). Cell engraftment (assessed by immunohistochemistry or bioluminescence imaging of luciferase-expressing cells), arterial re-endothelialisation (evaluated by immunohistochemical staining for rat endothelial cell antigen-1) and restriction of vascular smooth muscle cell proliferation in the neointima (double-staining of alpha-smooth muscle actin and phospho-histone H3) were greater when MSC sheets were applied than when MSC suspensions were used. In conclusion, MSC sheets exhibited better anti-stenotic and cell engraftment properties than MSC suspensions. MSC sheet transplantation from the adventitial side is a promising therapy for prevention of arterial restenosis. |
DOI | 10.1089/ten.TEA.2018.0030 |
PMID | 29724149 |