ホンマ ジユン   Homma Jun
  本間 順
   所属   研究施設 研究施設
   職種   助教
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Mesenchymal stem cell sheets exert anti-stenotic effects in a rat arterial injury model.
掲載誌名 正式名:Tissue engineering. Part A.
略  称:Tissue Eng Part A
ISSNコード:19373341/1937335X
掲載区分国外
巻・号・頁 24(19-20),1545-1553頁
著者・共著者 HOMMA Jun†, SEKINE Hidekazu*, MATSUURA Katsuhisa, SHIMIZU Tatsuya
発行年月 2018/10
概要 Restenosis after catheter or surgical intervention substantially affects the prognosis of arterial occlusive disease. Mesenchymal stem cells (MSCs) may have anti-stenotic effects on injured arteries. MSC transplantation from the adventitial side of an artery is safer than endovascular transplantation but has not been extensively examined. In this study, a rat model of femoral artery injury was used to compare the anti-stenotic effects of transplanted cell sheets and transplanted cell suspensions. Rat adipose-derived stem cells (ASCs) were used as the source of MSCs. For both cell sheets and suspensions, 6×106 MSCs were transplanted on the day of arterial injury. MSC sheets attenuated neointimal hyperplasia more than MSC suspensions (intima-to-media ratio in haematoxylin/eosin-stained sections: 0.55±0.13 vs. 1.14±0.12; P<0.05). Cell engraftment (assessed by immunohistochemistry or bioluminescence imaging of luciferase-expressing cells), arterial re-endothelialisation (evaluated by immunohistochemical staining for rat endothelial cell antigen-1) and restriction of vascular smooth muscle cell proliferation in the neointima (double-staining of alpha-smooth muscle actin and phospho-histone H3) were greater when MSC sheets were applied than when MSC suspensions were used. In conclusion, MSC sheets exhibited better anti-stenotic and cell engraftment properties than MSC suspensions. MSC sheet transplantation from the adventitial side is a promising therapy for prevention of arterial restenosis.
DOI 10.1089/ten.TEA.2018.0030
PMID 29724149