ナカヤマ ヒサコ   Hisako Nakayama
  中山 寿子
   所属   医学部 医学科
   職種   助教
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Microglia permit climbing fiber elimination by promoting GABAergic inhibition in the developing cerebellum
掲載誌名 正式名:Nature Communications.
略  称:Nat Commun
ISSNコード:20411723
掲載区分国外
巻・号・頁 9(1),2830頁
著者・共著者 Hisako Nakayama†, Manabu Abe†, Chie Morimoto, Tadatune Iida, Shigeo Okabe, Kenji Sakimura, Kouichi Hashimoto*
発行年月 2018/07
概要 Circuit refinement during postnatal development is finely regulated by neuron–neuron interactions. Recent studies suggest participation of microglia in this process but it is unclear how microglia cooperatively act with neuronal mechanisms. To examine roles of microglia, we ablate microglia by microglia-selective deletion of colony-stimulating factor 1 receptor (Csf1r) by crossing floxed-Csf1r and Iba1-iCre mice (Csf1r-cKO). In Csf1r-cKO mice, refinement of climbing fiber (CF) to Purkinje cell (PC) innervation after postnatal day 10 (P10)–P12 is severely impaired. However, there is no clear morphological evidence suggesting massive engulfment of CFs by microglia. In Csf1r-cKO mice, inhibitory synaptic transmission is impaired and CF elimination is restored by diazepam, which suggests that impairment of CF elimination is caused by a defect of GABAergic inhibition on PCs, a prerequisite for CF elimination. These results indicate that microglia primarily promote GABAergic inhibition and secondarily facilitate the mechanism for CF elimination inherent in PCs.
DOI 10.1038/s41467-018-05100-z
PMID 30026565