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Hisako Nakayama
Department School of Medicine, School of Medicine Position Associate Professor |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | CAPS1 RNA Editing Promotes Dense Core Vesicle Exocytosis. |
| Journal | Formal name：Cell Reports Abbreviation：Cell Rep ISSN code：22111247 |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 17(8),pp.2004-2014 |
| Author and coauthor | MIYAKE Kotaro†, OHTA Toshio, NAKAYAMA Hisako, DOE Nobutaka, TERAO Yuri, OIKI Eiji, NAGAMOTO Izumi, YAMASHITA Yui, ABE Takaya, NISHIKURA Kazuko, KUMANOGOH Atsushi, HASHIMOTO Kouichi, KAWAHARA Yukio* |
| Publication date | 2016/12 |
| Summary | Calcium-dependent activator protein for secretion 1 (CAPS1) plays a distinct role in the priming step of dense core vesicle (DCV) exocytosis. CAPS1 pre-mRNA is known to undergo adenosine-to-inosine RNA editing in its coding region, which results in a glutamate-to-glycine conversion at a site in its C-terminal region. However, the physiological significance of CAPS1 RNA editing remains elusive. Here, we created mutant mice in which edited CAPS1 was solely expressed. These mice were lean due to increased energy expenditure caused by physical hyperactivity. Electrophysiological and biochemical analyses demonstrated that the exocytosis of DCVs was upregulated in the chromaffin cells and neurons of these mice. Furthermore, we showed that edited CAPS1 bound preferentially to the activated form of syntaxin-1A, a component of the exocytotic fusion complex. These findings suggest that RNA editing regulates DCV exocytosis in vivo, affecting physical activity. |
| DOI | 10.1016/j.celrep.2016.10.073 |
| PMID | 27851964 |