ナカヤマ ヒサコ   Hisako Nakayama
  中山 寿子
   所属   医学部 医学科
   職種   助教
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 CAPS1 RNA Editing Promotes Dense Core Vesicle Exocytosis.
掲載誌名 正式名:Cell Reports
略  称:Cell Rep
ISSNコード:22111247
掲載区分国外
巻・号・頁 17(8),2004-2014頁
著者・共著者 MIYAKE Kotaro†, OHTA Toshio, NAKAYAMA Hisako, DOE Nobutaka, TERAO Yuri, OIKI Eiji, NAGAMOTO Izumi, YAMASHITA Yui, ABE Takaya, NISHIKURA Kazuko, KUMANOGOH Atsushi, HASHIMOTO Kouichi, KAWAHARA Yukio*
発行年月 2016/12
概要 Calcium-dependent activator protein for secretion 1 (CAPS1) plays a distinct role in the priming step of dense core vesicle (DCV) exocytosis. CAPS1 pre-mRNA is known to undergo adenosine-to-inosine RNA editing in its coding region, which results in a glutamate-to-glycine conversion at a site in its C-terminal region. However, the physiological significance of CAPS1 RNA editing remains elusive. Here, we created mutant mice in which edited CAPS1 was solely expressed. These mice were lean due to increased energy expenditure caused by physical hyperactivity. Electrophysiological and biochemical analyses demonstrated that the exocytosis of DCVs was upregulated in the chromaffin cells and neurons of these mice. Furthermore, we showed that edited CAPS1 bound preferentially to the activated form of syntaxin-1A, a component of the exocytotic fusion complex. These findings suggest that RNA editing regulates DCV exocytosis in vivo, affecting physical activity.
DOI 10.1016/j.celrep.2016.10.073
PMID 27851964