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Hisako Nakayama
Department School of Medicine, School of Medicine Position Associate Professor |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | Spike timing-dependent selective strengthening of single climbing fibre inputs to Purkinje cells during cerebellar development. |
| Journal | Formal name：Nature Communications Abbreviation：Nat Commun ISSN code：20411723 |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 4,pp.2732 |
| Author and coauthor | KAWAMURA Yoshinobu†, NAKAYAMA Hisako, HASHIMOTO Kouichi, SAKIMURA Kenji, KITAMURA Kazuo, KANO Masanobu* |
| Authorship | 2nd author |
| Publication date | 2013/11 |
| Summary | Shaping functional neural circuits in developing brain involves activity-dependent refinement of early-formed redundant synapses. In the developing cerebellum, a one-to-one connection between a climbing fibre (CF) and a Purkinje cell (PC) is established by selective strengthening of a single CF followed by elimination of surplus CFs. Here we investigate developmental changes in CF-mediated responses in PCs by using in vivo whole-cell recordings and two-photon Ca2+ imaging. We show that each neonatal PC receives temporally clustered inputs from multiple CFs and temporal integration of these inputs is required to induce burst spiking and Ca2+ rise in PCs. Importantly, a single CF input closest to PC’s spike output is selectively strengthened during postnatal development. This spike timing-dependent selective strengthening is much less prominent in PC-selective P/Q-type voltage-dependent Ca2+ channel knockout mice. Thus, spike timing- and Ca2+-dependent plasticity appears to underlie the selection of a single ‘winner’ CF and the establishment of mature CF–PC connections. |
| DOI | 10.1038/ncomms3732. |
| PMID | 24225482 |