ナカヤマ ヒサコ   Hisako Nakayama
  中山 寿子
   所属   医学部 医学科
   職種   助教
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Spike timing-dependent selective strengthening of single climbing fibre inputs to Purkinje cells during cerebellar development.
掲載誌名 正式名:Nature Communications
略  称:Nat Commun
ISSNコード:20411723
掲載区分国外
巻・号・頁 4,2732頁
著者・共著者 KAWAMURA Yoshinobu†, NAKAYAMA Hisako, HASHIMOTO Kouichi, SAKIMURA Kenji, KITAMURA Kazuo, KANO Masanobu*
発行年月 2013/11
概要 Shaping functional neural circuits in developing brain involves activity-dependent refinement of early-formed redundant synapses. In the developing cerebellum, a one-to-one connection between a climbing fibre (CF) and a Purkinje cell (PC) is established by selective strengthening of a single CF followed by elimination of surplus CFs. Here we investigate developmental changes in CF-mediated responses in PCs by using in vivo whole-cell recordings and two-photon Ca2+ imaging. We show that each neonatal PC receives temporally clustered inputs from multiple CFs and temporal integration of these inputs is required to induce burst spiking and Ca2+ rise in PCs. Importantly, a single CF input closest to PC’s spike output is selectively strengthened during postnatal development. This spike timing-dependent selective strengthening is much less prominent in PC-selective P/Q-type voltage-dependent Ca2+ channel knockout mice. Thus, spike timing- and Ca2+-dependent plasticity appears to underlie the selection of a single ‘winner’ CF and the establishment of mature CF–PC connections.
DOI 10.1038/ncomms3732.
PMID 24225482