ナカヤマ ヒサコ   Hisako Nakayama
  中山 寿子
   所属   医学部 医学科
   職種   助教
論文種別 総説
言語種別 英語
査読の有無 査読あり
表題 Calcium-dependent regulation of climbing fibre synapse elimination during postnatal cerebellar development.
掲載誌名 正式名:The Journal of physiology
略  称:J Physiol
ISSNコード:00223751/14697793
掲載区分国外
巻・号・頁 591(13),3151-3158頁
著者・共著者 KANO Masanobu†, NAKAYAMA Hisako, HASHIMOTO Kouichi, KITAMURA Kazuo, SAKIMURA Kenji, WATANABE Masahiko*
発行年月 2013/07
概要 Functional neural circuit formation during postnatal development involves massive elimination of early-formed redundant synapses and strengthening of necessary synaptic connections. In the cerebellum, one-to-one connection from a climbing fibre (CF) to a Purkinje cell (PC) is established through four distinct phases: (1) strengthening of a single CF among multiple CFs in each PC at postnatal age P3–P7 days, (2) translocation of a single strengthened CF to PC dendrites from around P9, (3) early-phase (P7 to around P11) and (4) late-phase (around P12–P17) elimination of weak CF synapses from PC somata. Mice with PC-selective deletion of the P/Q-type voltage-dependent Ca2+ channel (VDCC) exhibit severe defects in strengthening of single CFs, dendritic translocation of single CFs and CF elimination from P7. In contrast, mice with a mutation of a single allele for the GABA synthesizing enzyme GAD67 show selective impairment of CF elimination from P10. Electrophysiological and Ca2+ imaging data suggest that GABAA receptor-mediated inhibition onto PC somata from putative basket cells influences CF-induced Ca2+ transients and regulates elimination of redundant CF synapses from PC somata at P10–P16. Thus, regulation of Ca2+ influx to PCs through VDCCs is crucial for the four phases of CF synapse elimination during postnatal development.
DOI 10.1113/jphysiol.2012.248252.
PMID 23359672