コバヤシ ジユン   KOBAYASHI Jun
  小林 純
   所属   研究施設 研究施設
   職種   講師
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Shear stress-dependent cell detachment from temperature-responsive cell culture surfaces in a microfluidic device
掲載誌名 正式名:Biomaterials
ISSNコード:0142-9612
巻・号・頁 33(30),7405-7411頁
著者・共著者 Tang Zhonglan†, Akiyama Yoshikatsu, Itoga Kazuyoshi, Kobayashi Jun, Yamato Masayuki, Okano Teruo*
発行年月 2012/10
概要 A new approach to quantitatively estimate the interaction between cells and material has been proposed by using a microfluidic system, which was made of poly(dimethylsiloxane) (PDMS) chip bonding on a temperature-responsive cell culture surface consisted of poly(N-isopropylacrylamide) (PIPAAm) grafted tissue culture polystyrene (TCPS) (PIPAAm-TCPS) having five parallel test channels for cell culture. This construction allows concurrently generating five different shear forces to apply to cells in individual microchannels having various resistance of each channel and simultaneously gives an identical cell incubation condition to all test channels. NIH/3T3 mouse fibroblast cells (MFCs) and bovine aortic endothelial cells (BAECs) were well adhered and spread on all channels of PIPAAm-TCPS at 37 oC. In our previous study, reducing culture temperature below the lower critical solution temperature (LCST) of PIPAAm (32 oC), cells detach themselves from hydrated PIPAAm grafted surfaces spontaneously. In this study, cell detachment process from hydrated PIPAAm-TCPS was promoted by shear forces applied to cells in microchannels. Shear stress-dependent cell detachment process from PIPAAm-TCPS was evaluated at various shear stresses. Either MFCs or BAECs in the microchannel with the strongest shear stress were found to be detached from the substrate more quickly than those in other microchannels. A cell transformation rate constant Ct and an intrinsic cell detachment rate constant k0 were obtained through studying the effect of shear stress on cell detachment with a peeling model. The proposed device and quantitative analysis could be used to assess the possible interaction between cells and PIPAAm layer with a potential application to design a cell sheet culture surface for tissue engineering.
DOI 10.1016/j.biomaterials.2012.06.077