Ayako Nakamura-Ishizu
   Department   School of Medicine, School of Medicine
   Position   Professor and Division head
Article types Original article
Language English
Peer review Peer reviewed
Title Sphere formation restores and confers hair-inducing capacity in cultured mesenchymal cells.
Journal Formal name:Experimental dermatology
Abbreviation:Exp Dermatol
ISSN code:16000625/09066705
Domestic / ForeginForegin
Volume, Issue, Page 20(8),pp.679-81
Author and coauthor Shimizu Ruka, Okabe Keisuke, Kubota Yoshiaki, Nakamura-Ishizu Ayako, Nakajima Hideo, Kishi Kazuo
Publication date 2011/08
Summary Interactions between epithelial and dermal cells are essential for hair follicle morphogenesis and maintenance. In experimental trials of hair regeneration, isolated dermal cells have been shown to possess hair-inducing capacity. However, dermal cells lose this potential immediately after cultivation. Sphere-forming multipotent cells derived from the dermis possess hair-inducing capacity. These previous findings raise the question of whether hair-inducing capacity depends on the identity as dermal cells or the process of sphere formation. To address this issue, we compared the in vitro and in vivo characteristics of two-dimensionally cultured or thereafter sphere formation-induced dermal and lung mesenchymal cells. We show that sphere-forming mesenchymal cells exhibited higher expression of Wnt signalling genes. Sphere-forming cells but not two-dimensionally cultured cells possessed in vivo hair-inducing capacity. These data suggest that various mesenchymal cells attain hair-inducing capacity through the process of sphere formation.
DOI 10.1111/j.1600-0625.2011.01281.x
PMID 21521371