コモイケ ユウタ   Komoike Yuta
  蒋池 勇太
   所属   医学部 医学科
   職種   准教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Adaptive changes in TEF-1 gene expression during cold acclimation in the medaka.
掲載誌名 正式名:Zoological science
略  称:Zoolog Sci
ISSNコード:(0289-0003)0289-0003(Linking)
巻・号・頁 23(10),903-8頁
著者・共著者 Yamasaki Yukihiko, Komoike Yuta, Higashinakagawa Toru
発行年月 2006/10
概要 How animals adaptively respond to a cold or hot environment has been questioned for a long time. Recently, with the aid of microarray analysis, various temperature-sensitive genes have been identified in several species. However, a definitive hypothesis regarding the mechanism of adaptation has not been proposed. In the present study, we surveyed, in medaka (Oryzias latipes), genes for which the level of expression changes depending on the surrounding temperature. A messenger RNA differential display of medaka muscle total RNA revealed one such gene encoding transcription enhancer factor-1 (TEF-1). In medaka muscle, the TEF-1 gene produces two splicing variants, TEF-1A and TEF-1B mRNAs. During cold acclimation, the mRNA level of TEF-1A decreased, whereas that of TEF-1B increased. We also found that three putative downstream genes of TEF-1, two for myosin heavy chain (MyHC) and one for troponin T (TnT), a specific group of muscle proteins, were transcribed in a temperature-dependent manner. These results suggest that the transcription of MyHC and/or TnT is regulated by TEF-1 and that these molecules participate in muscle reconstruction during temperature adaptation in fish.
DOI 10.2108/zsj.23.903
PMID 17116993