コモイケ ユウタ   Komoike Yuta
  蒋池 勇太
   所属   医学部 医学科
   職種   准教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Identification of a nuclear localization signal in mouse polycomb protein, M33.
掲載誌名 正式名:Zoological science
略  称:Zoolog Sci
ISSNコード:(0289-0003)0289-0003(Linking)
巻・号・頁 23(9),785-91頁
著者・共著者 Hirose Sayako, Komoike Yuta, Higashinakagawa Toru
発行年月 2006/09
概要 The mouse Polycomb group (PcG) protein M33 forms nuclear complexes with the products of other PcG members and maintains repressed states of developmentally important genes, including homeotic genes. In this context, nuclear localization is a prerequisite for M33 to exert its function. However, we previously found that M33 in mouse liver shuttles dynamically between the nucleus and the cytoplasm, depending on the proliferative states of cells, coupled with phosphorylation and dephosphorylation of M33 protein. To understand the mechanism and significance of this phenomenon, we identified the functional nuclear localization signal (NLS) of M33 protein. Deletion mutants that lack a particular one of three putative NLS motifs failed to localize in the nucleus. Green fluorescent protein (GFP) fused to this motif specifically localized in the nucleus. We conclude that this amino-acid stretch in M33 acts as the functional NLS for this protein.
DOI 10.2108/zsj.23.785
PMID 17043400