コモイケ ユウタ   Komoike Yuta
  蒋池 勇太
   所属   医学部 医学科
   職種   准教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Zebrafish Polycomb group gene ph2alpha is required for epiboly and tailbud formation acting downstream of FGF signaling.
掲載誌名 正式名:Biochemical and biophysical research communications
略  称:Biochem Biophys Res Commun
ISSNコード:(0006-291X)0006-291X(Linking)
巻・号・頁 328(4),858-66頁
著者・共著者 Komoike Yuta, Kawamura Akinori, Shindo Norihisa, Sato Chie, Satoh Junichi, Shiurba Robert, Higashinakagawa Toru
発行年月 2005/03
概要 We analyzed Polycomb group gene ph2alpha functionally in zebrafish embryos by a gene knock-down procedure using morpholino antisense oligos. Inhibition of ph2alpha message translation resulted in abnormal epibolic movements as well as a thick tailbud or incomplete covering of the yolk plug. At the 24hpf stage, morphants had short trunks and tails, phenotypes similar to those with disturbances in FGF signaling. Accordingly, we looked at the effects of ph2alpha expression upstream and downstream of the FGF pathway. Treatment with SU5402, an inhibitor of Fgfrs, or injection of dominant-negative Fgfr1 DNA markedly reduced ph2alpha expression in the tailbud. In addition, cells expressing mRNAs for no tail, spadetail, myoD, and papc, which are involved in FGF-related development of posterior mesoderm, were distributed abnormally. Collectively, the data argue that ph2alpha is required for epiboly and tailbud formation, acting downstream of the FGF signaling pathway.
DOI 10.1016/j.bbrc.2005.01.031
PMID 15707958