大野　秀樹    Department   School of Medicine(Tokyo Women's Medical University Adachi Medical Center), School of Medicine    Position   Assistant Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	C2PA is a nuclear protein implicated in the heat shock response
Journal	Formal name：J Cell Biochem ISSN code：0730-2312 (Print) 0730-2312 (Linking)
Domestic / Foregin	Foregin
Volume, Issue, Page	87,pp.65-74
Author and coauthor	Hirabayashi, S., Ohno, H., Iida, J., Hata, Y.
Authorship	2nd author
Publication date	2002
Summary	C2PA is a protein of unknown function that is expressed in spermatocytes. PDZ-RGS3 is a signaling molecule whose PDZ domain binds Ephrin-B2 and mediates reverse signaling of this protein. C2PA and PDZ-RGS3 have identical PDZ domains. To explore the function of C2PA, we compared it with PDZ-RGS3 with respect to tissue distribution, subcellular localization, and biochemistry. C2PA is expressed only in testis, whereas PDZ-RGS3 is expressed in various tissues including brain, heart, lung, liver, spleen, kidney, small intestine, skeletal muscles, and testis. These proteins also differ in their subcellular distribution, in that PDZ-RGS3 is cytosolic while C2PA is exclusively nuclear. C2PA is distributed diffusely in the nucleus and forms a few foci at 37 degrees C. However, when cells are exposed to 42 degrees C, the number of C2PA foci is increased. These heat shock-induced foci colocalize with CREB-binding protein and heat shock factor-1. In contrast, the distribution of PDZ-RGS3 does not change during heat stress. When overexpressed, C2PA induces heat shock response element (HSE)-dependent gene transcription, whereas PDZ-RGS3 does not. These data suggest that the function of C2PA is distinct from that of PDZ-RGS3, and that C2PA may be involved in the heat shock response in testis.
DOI	10.1002/jcb.10279
Document No.	12210723