猪狩　勝則    Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine    Position   Endowed Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Dermatan sulfate in the synovial fluid of patients with knee osteoarthritis.
Journal	Formal name：Modern rheumatology Abbreviation：Mod Rheumatol ISSN code：(1439-7595)1439-7595(Linking)
Volume, Issue, Page	17(4),pp.301-305
Author and coauthor	MOMOHARA Shigeki, OKADA Naoyuki, IKARI Katsunori, MIZUNO Shoji, OKAMOTO Hiroshi
Publication date	2007
Summary	Biochemical factors play an important role in osteoarthritis (OA) pathogenesis. The purpose of this study is to clarify whether the dermatan sulfate (DS) levels in the synovial fluid of patients with knee OA are related to residual cartilage. Synovial fluid was obtained from 51 OA patients. Knee radiographs were evaluated with the Kellgren-Lawrence (K/L) grading scale. The levels of the following disaccharides were measured by high-performance liquid chromatography (HPLC): DS (DSDeltaDi4S), chondroitin 6-sulfate (CSDeltaDi6S), and chondroitin 4-sulfate (CSDeltaDi4S). The concentration of cartilage oligomeric matrix protein (COMP) was measured by a sandwich ELISA. The levels of DSDeltaDi4S in Grades 0 and I OA were significantly higher than levels in Grade II (P = 0.0458), Grade III (P < 0.0001) and Grade IV (P < 0.0001), and we found strong relationships between the levels of DSDeltaDi4S and those of CSDeltaDi6S (P < 0.0001, r = 0.705), CSDeltaDi4S (P < 0.0001, r = 0.750), and COMP (P < 0.0001, r = 0.699). We conclude that the presence of DSDeltaDi4S reflects proteoglycan metabolism in the residual articular cartilage of OA patients. This suggests that metabolism of the small leucine-rich repeat proteoglycans decorin and biglycan, which contain chains of DSDeltaDi4S, is similar to that of aggrecan.
DOI	10.1007/s10165-007-0594-7
PMID	17694263