SHIMIZU MIKIKO
   Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine
   Position   Assistant Professor
Article types Original article
Language English
Peer review Peer reviewed
Title Remote ischemic preconditioning protects human neural stem cells from oxidative stress.
Journal Formal name：Apoptosis : an international journal on programmed cell death
Abbreviation：Apoptosis
ISSN code：13608185/1573675X
Domestic / ForeginForegin
Publisher Springer Nature Switzerland AG
Volume, Issue, Page 22(11),pp.1353-1361
Author and coauthor Motomura Ayako†, Shimizu Mikiko, Kato Akira, Motomura Kazuya, Yamamichi Akane, Koyama Hiroko, Ohka Fumiharu, Nishikawa Tomohide, Nishimura Yusuke, Hara Masahito, Fukuda Tetsuya, Bando Yasuhiko, Nishimura Toshihide, Wakabayashi Toshihiko, Natsume Atsushi*
Publication date 2017/11
Summary In previous clinical trials, we showed that remote ischemic preconditioning (rIPC) reduced myocardial damage in children undergoing treatment for congenital heart defects and postoperative renal failure in patients undergoing abdominal aortic aneurysm surgery. In rabbit experiments, pre-treatment with plasma and plasma dialysate (obtained using 15-kDa cut-off dialysis membrane) from donor rabbits subjected to rIPC similarly protected against cardiac infarction. However, the protective substances containing in rIPC plasma have been unknown. In the present study, we showed that rIPC plasma exerted anti-apoptotic and anti-oxidative effects on human neural stem cells under oxygen glucose deprivation (OGD) that mimics brain ischemia. Additionally, we applied the sample to the liquid chromatography integrated with mass spectrometry to identify candidate key molecules in the rIPC plasma and determine its role in protecting neural stem cells from OGD-induced cell death. Thioredoxin increased significantly after rIPC compared to pre-IPC. Pretreatment with thioredoxin, the antioxidant protein, markedly protected human neural stem cells from OGD-induced cell death. The effect ofthioredoxin on brain ischemia in animals should be further evaluated. However, the present study first evaluated the effect of rIPC in the ischemic cellular model.
DOI 10.1007/s10495-017-1425-8
PMID 28951984