イシド　ミキコ   SHIMIZU MIKIKO   石戸　美妃子    所属   医学部 医学科（東京女子医科大学病院）    職種   講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Remote ischemic preconditioning protects human neural stem cells from oxidative stress.
掲載誌名	正式名：Apoptosis : an international journal on programmed cell death 略　 称：Apoptosis ISSNコード：13608185/1573675X
掲載区分	国外
出版社	Springer Nature Switzerland AG
巻・号・頁	22(11),pp.1353-1361
著者・共著者	Motomura Ayako†, Shimizu Mikiko, Kato Akira, Motomura Kazuya, Yamamichi Akane, Koyama Hiroko, Ohka Fumiharu, Nishikawa Tomohide, Nishimura Yusuke, Hara Masahito, Fukuda Tetsuya, Bando Yasuhiko, Nishimura Toshihide, Wakabayashi Toshihiko, Natsume Atsushi*
発行年月	2017/11
概要	In previous clinical trials, we showed that remote ischemic preconditioning (rIPC) reduced myocardial damage in children undergoing treatment for congenital heart defects and postoperative renal failure in patients undergoing abdominal aortic aneurysm surgery. In rabbit experiments, pre-treatment with plasma and plasma dialysate (obtained using 15-kDa cut-off dialysis membrane) from donor rabbits subjected to rIPC similarly protected against cardiac infarction. However, the protective substances containing in rIPC plasma have been unknown. In the present study, we showed that rIPC plasma exerted anti-apoptotic and anti-oxidative effects on human neural stem cells under oxygen glucose deprivation (OGD) that mimics brain ischemia. Additionally, we applied the sample to the liquid chromatography integrated with mass spectrometry to identify candidate key molecules in the rIPC plasma and determine its role in protecting neural stem cells from OGD-induced cell death. Thioredoxin increased significantly after rIPC compared to pre-IPC. Pretreatment with thioredoxin, the antioxidant protein, markedly protected human neural stem cells from OGD-induced cell death. The effect ofthioredoxin on brain ischemia in animals should be further evaluated. However, the present study first evaluated the effect of rIPC in the ischemic cellular model.
DOI	10.1007/s10495-017-1425-8
PMID	28951984