Gaku MASUDA    Department   School of Medicine, School of Medicine    Position   Assistant Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Reduction in Plasmodium falciparum Pfk13 and pfg377 allele diversity through time in southern Vietnam
Journal	Formal name：Tropical Medicine and Health ISSN code：13488945/13494147
Domestic / Foregin	Domestic
Publisher	(一社)日本熱帯医学会
Volume, Issue, Page	50,pp.1 of 7-7 of 7
International coauthorship	International coauthorship
Author and coauthor	Nguyen Quang Thieu†, Vu Duc Chinh, Truong Van Hanh, Nguyen Van Dung, Takagi Hidekazu, Annoura Takeshi, Kawai Satoru, Masuda Gaku, Nguyen Van Tuan, Vu Viet Hung, Nakazawa Shusuke, Culleton Richard, Nguyen Thi Huong Binh, Maeno Yoshimasa*
Publication date	2022
Summary	Method This study was conducted in Bu Gia Map commune, Binh Phuoc province, Vietnam, from May 2018 to January 2019. Twenty-four samples from 2018 to 2019, 30 from 2003, 24 from 2012 and 32 from 2015 were analysed. Malaria-infected human blood was collected by finger-prick and used for molecular analysis. A nested-PCR targeting the small subunit ribosomal RNA gene was used for Plasmodium species identification, followed by amplification and nucleotide sequencing of Pfk13 and region 3 of pfg377. Archived blood samples collected in the same region in 2012 and 2015 were also analysed as above for comparison. Results The genetic diversity of Pfk13 and pfg377 was lower in 2018–2019 compared to 2012 and 2015. The number of distinct Pfk13 mutants decreased from three in 2012 and 2015, P553L, V568G and C580Y, to one, C580Y in 2018–2019. In 2018–2019, the frequency of C580Y mutant strains was 71% (17/24 isolates). All samples were wild type in 2003. In 2012 and 2015, there were single-strain infections as well as co-infections with two mutant strains or with mutant and wild strains, whereas there were no co-infections in 2018. pfg377 allele diversity decreased from five alleles in 2012 to two alleles in 2018–2019. Conclusion The genetic diversity of P. falciparum was reduced at the two genetic loci surveyed in this study, Pfk13 and pfg377. In the case of the former gene, we observed an increase in the prevalence of parasites carrying the C580Y gene, known to confer reduced susceptibility to ACTs. The reduction in the diversity of pfg377 may be linked to the clonal expansion of parasite strains carrying the C580Y mutation, leading to an overall reduction in parasite genetic diversity across the population.
DOI	10.1186/s41182-022-00409-4
Document No.	W603390018<Pre 医中誌>