マスダ ガク   Gaku MASUDA
  益田 岳
   所属   医学部 医学科
   職種   助教
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Reduction in Plasmodium falciparum Pfk13 and pfg377 allele diversity through time in southern Vietnam.
掲載誌名 正式名:Tropical medicine and health
略  称:Trop Med Health
ISSNコード:13488945/13488945
掲載区分国外
巻・号・頁 50(1),pp.19
著者・共著者 Thieu Nguyen Quang, Chinh Vu Duc, Van Hanh Truong, Van Dung Nguyen, Takagi Hidekazu, Annoura Takeshi, Kawai Satoru, Masuda Gaku, Van Tuan Nguyen, Hung Vu Viet, Nakazawa Shusuke, Culleton Richard, Binh Nguyen Thi Huong, Maeno Yoshimasa
発行年月 2022/03
概要 BACKGROUND:Plasmodium falciparum has acquired resistance to artemisinin in Southeast Asia, with mutations in the P. falciparum Kelch-13 (Pfk13) gene associated with the resistance phenotype. The widespread use of Artemisinin-based combination therapy (ACT)s in Southeast Asia has led to the selection and spread of parasites carrying mutations in Pfk13. We characterised the allele diversity of Pfk13 and pfg377, an artemisinin-resistance neutral polymorphic gene, in parasite DNA extracted human blood from in southern Vietnam in 2003, 2012, 2015 and 2018.METHOD:This study was conducted in Bu Gia Map commune, Binh Phuoc province, Vietnam, from May 2018 to January 2019. Twenty-four samples from 2018 to 2019, 30 from 2003, 24 from 2012 and 32 from 2015 were analysed. Malaria-infected human blood was collected by finger-prick and used for molecular analysis. A nested-PCR targeting the small subunit ribosomal RNA gene was used for Plasmodium species identification, followed by amplification and nucleotide sequencing of Pfk13 and region 3 of pfg377. Archived blood samples collected in the same region in 2012 and 2015 were also analysed as above for comparison.RESULTS:The genetic diversity of Pfk13 and pfg377 was lower in 2018-2019 compared to 2012 and 2015. The number of distinct Pfk13 mutants decreased from three in 2012 and 2015, P553L, V568G and C580Y, to one, C580Y in 2018-2019. In 2018-2019, the frequency of C580Y mutant strains was 71% (17/24 isolates). All samples were wild type in 2003. In 2012 and 2015, there were single-strain infections as well as co-infections with two mutant strains or with mutant and wild strains, whereas there were no co-infections in 2018. pfg377 allele diversity decreased from five alleles in 2012 to two alleles in 2018-2019.CONCLUSION:The genetic diversity of P. falciparum was reduced at the two genetic loci surveyed in this study, Pfk13 and pfg377. In the case of the former gene, we observed an increase in the prevalence of parasites carryi
DOI 10.1186/s41182-022-00409-4
PMID 35232492