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野口 岳春
Department School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine Position Assistant Professor |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | Rapid Flow Cytometry of Gastrointestinal Stromal Tumours Closely Matches the Modified Fletcher Classification. |
| Journal | Formal name：Anticancer research Abbreviation：Anticancer Res ISSN code：17917530/02507005 |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 41(1),pp.131-136 |
| Author and coauthor | TANIGUCHI Kiyoaki†, SUZUKI Akane, SERIZAWA Akiko, KOTAKE Sho, ITO Shunichi, SUZUKI Kazuomi, YAMADA Takuji, NOGUCHI Takeharu, AMANO Kunihiko, OTA Masaho, MURAGAKI Yoshihiro, YAMAMOTO Masakazu |
| Publication date | 2021/01/10 |
| Summary | AIM:We aimed to develop a rapid, simple procedure and an algorithm for quantitative analysis and classification of the metastatic risk of gastrointestinal stromal tumours (GIST) for clinical use.MATERIALS AND METHODS:Eighteen specimens from laparoscopic local gastrectomy were assessed by flow cytometry. We devised a new risk classification for GIST by combining flow cytometry parameters with tumour size and evaluated whether the combined parameters correlated with the modified Fletcher risk classification.RESULTS:We found a significant correlation between clinical prognostic factors (mitotic count and Ki-67 labelling index) and the flow cytometry parameters DNA ploidy, DNA index and S-phase fraction. The combined parameters established from tumour size and the flow cytometry parameters showed a high correlation with the modified Fletcher risk classification (p=0.0064). Flow cytometry had to be performed for approximately 10 minutes to determine the metastatic risk.CONCLUSION:Rapid flow cytometry parameters can classify risk without the need for histological analysis. |
| DOI | 10.21873/anticanres.14758 |
| PMID | 33419806 |