Yoko Kawase-Koga    Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine    Position   Professor and Division head
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Properties of growth and molecular profiles of rat progenitor cells from ciliary epithelium.
Journal	Formal name：Experimental eye research Abbreviation：Exp Eye Res ISSN code：00144835/00144835
Domestic / Foregin	Foregin
Volume, Issue, Page	82(3),pp.471-8
Author and coauthor	Yanagi Yasuo, Inoue Yuji, Kawase Yoko, Uchida Saiko, Tamaki Yasuhiro, Araie Makoto, Okochi Hitoshi
Publication date	2006/03
Summary	Recent studies have demonstrated that multipotent retinal stem or progenitor cells can be isolated from the ciliary epithelium (CE) of the eye using a neurosphere culture. In this study, we investigated the properties of growth and differentiation, and molecular profiles of rat adult ciliary epithelium (CE)-derived retinal progenitors and forebrain (FB) derived neurospheres. Under clonogenic culture conditions, we found that the CE-derived neurospheres contained fewer undifferentiated cells compared with the FB-derived neurospheres, and that CE-derived neurospheres initially expressed the set of Notch pathway molecules genes including Notch 1 and Delta 1, HES-1 and HES-5, but partially lose their expression after passaging. Furthermore, we found that the CE-derived neurospheres did not express several markers for in vivo embryonic retinal progenitors. Additionally, when the eye was divided into four subregions along its dorsoventral and nasotemporal axes and progenitor cells were obtained from the subregions, the progenitor cells did not express the subregion specific transcription factors, suggesting that subregional specificity is not maintained in vitro. Together, our results demonstrate that CE-derived progenitor cells may have intrinsic limitations in the production of cell types.
DOI	10.1016/j.exer.2005.08.005
PMID	16198338