アキザワ ヨシカ
  秋澤 叔香
   所属   医学部 医学科(東京女子医科大学病院)
   職種   講師
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Possible Application of Ascites-infiltrating Gamma-delta T Cells for Adoptive Immunotherapy.
掲載誌名 正式名:Anticancer research
略  称:Anticancer Res
ISSNコード:17917530/02507005
掲載区分国外
巻・号・頁 38(7),pp.4327-4331
著者・共著者 Abe Yuki†, Kobayashi Hirohito*, Akizawa Yoshika, Ishitani Ken, Hashimoto Kazunori, Matsui Hideo
発行年月 2018/07
概要 BACKGROUND/AIM:Malignant ascites contain many tumour-infiltrating lymphocytes. γδ T cells with antitumour activity have attracted attention as effector cells in cancer immunotherapy. Vδ2+ T cells were cultured from peripheral blood mononuclear cells (PBMCs) and ascites-infiltrating lymphocytes (AILs) to compare the differences in response to 2-methyl-3-butenyl-1-pyrophosphate (2M3B1-PP) and zoledronate (Zol) as antigens in vitro.MATERIALS AND METHODS:To expand Vδ2+ T cells from PBMCs and AILs from 29 patients with cancer, these cells were cultured and subjected to analysis.RESULTS:The proliferation rate of Vδ2+ T cells was higher in both PBMCs and AILs when cultured with Zol than with 2M3B1-PP. Although Vδ2+ T cells show a higher rate of expansion in AILs compared to PBMCs, the number of mixed tumour cells in ascites was decreased when cultured with Zol.CONCLUSION:Vδ2+ T cells in AILs are cytotoxic to tumour cells in ascites and may be considered in adoptive immunotherapy.
DOI 10.21873/anticanres.12732
PMID 29970569