サエキ タダシロウ
  佐伯 忠賜朗
   所属   医学部 医学科(東京女子医科大学病院)
   職種   講師
論文種別 原著
言語種別 英語
査読の有無 査読有り
表題 Establishment of an experimental ferret ocular hypertension model for the analysis of central visual pathway damage.
掲載誌名 正式名:Scientific reports
略  称:Sci Rep
ISSNコード:(2045-2322)2045-2322(Linking)
掲載区分国外
巻・号・頁 4,6501頁
著者・共著者 Fujishiro Takashi, Kawasaki Hiroshi, Aihara Makoto, Saeki Tadashiro, Ymagishi Reiko, Atarashi Takuya, Mayama Chihiro, Araie Makoto
発行年月 2014/10
概要 Glaucoma optic neuropathy (GON) is a condition where pathogenic intraocular pressure (IOP) results in axonal damage following retinal ganglion cell (RGC) death, and further results in secondary damage of the lateral geniculate nucleus (LGN). Therapeutic targets for glaucoma thus focus on both the LGN and RGC. However, the temporal and spatial patterns of degeneration and the mechanism of LGN damage have not been fully elucidated. Suitable and convenient ocular hypertension (OH) animal models with binocular vision comparable to that of monkeys are strongly needed. The ferret is relatively small mammal with binocular vision like humans - here we report on its suitability for investigating LGN. We developed a new method to elevate IOP by injection of cultured conjunctival cells into the anterior chamber to obstruct aqueous outflow. Histologically, cultured conjunctival cells successfully proliferated to occlude the angle, and IOP was elevated for 13 weeks after injection. Macroscopically, the size of the eye gradually expanded. Subsequent enlargement of optic nerve head cupping and atrophic damage of LGN projected from the OH eye were clearly observed by anterograde staining with cholera toxin B. We believe the ferret may be a promising OH model to investigate secondary degeneration of central nervous system including LGN.
DOI 10.1038/srep06501
PMID 25308730