KITAHARA Shuji    Department   Research Institutes and Facilities, Research Institutes and Facilities    Position   Associate Professor (Fixed Term)
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Alteration of angiogenic patterns on B16BL6 melanoma development promoted in Matrigel.
Journal	Formal name：Medical molecular morphology Abbreviation：Med Mol Morphol ISSN code：18601499/18601499
Domestic / Foregin	Foregin
Volume, Issue, Page	43(1),pp.26-36
Author and coauthor	Kitahara Shuji, Morikawa Shunichi, Shimizu Kazuhiko, Abe Hiroyuki, Ezaki Taichi
Publication date	2010/03
Summary	Because the progression and metastasis of solid tumors depend on their local microcirculation, we sought to characterize tumor angiogenesis three dimensionally in a highly metastatic mouse melanoma model, B16BL6 (B16), injected with Matrigel into the subcutis in the skin on the back of syngeneic C57BL/6 mice. We found that B16 with Matrigel grew significantly faster than B16 alone and had altered tumor angiogenesis. Tumor vessels apparently grew vigorously in the opposite direction of the tumor without invading the tumor mass until at least day 10 of injection. In addition, vascular branching resulted not only from sprouting as was seen in B16 without Matrigel but also from vascular splitting, either because of compression from outside the vessels or from septum formation by endothelial cells. This phenomenon was characteristic of B16 cells, but not of other tumor cells, including Lewis lung carcinoma and ASH-1 hybridoma cell lines, both of which were tested under the same conditions. The reduction in various angiogenic factors in Matrigel did not affect the angiogenic patterns and tumor growth. We hypothesize that tumor vessels may vigorously alter their angiogenic patterns in response to the local microenvironment.
DOI	10.1007/s00795-009-0481-8
PMID	20340003