MATSUURA Katsuhisa    Department   School of Medicine, School of Medicine    Position   Professor and Division head
Article types	Original article
Language	English
Peer review	Non peer reviewed
Title	Decellularized Organ-Derived Scaffold Is a Promising Carrier for Human Induced Pluripotent Stem Cells-Derived Hepatocytes.
Journal	Formal name：Cells Abbreviation：Cells ISSN code：20734409
Domestic / Foregin	Foregin
Volume, Issue, Page	11(8),pp.1258
Author and coauthor	KOJIMA Hideaki†, YAGI Hiroshi*, KUSHIGE Hiroko, TODA Yukiko, TAKAYAMA Kazuo, MASUDA Shinako, MORISAKU Toshinori, TSUCHIDA Tomonori, KURODA Kohei, HIRUKAWA Kazuya, INUI Jumpei, NISHI Kotaro, NAKANO Yutaka, TANAKA Masayuki, HORI Shutaro, HASEGAWA Yasushi, ABE Yuta, KIAGO Minoru, ADACHI Shungo, TOMI Masatoshi, MATSUURA Katsuhisa, MIZUGUCHI Hiroyuki, KITAGAWA Yuko
Publication date	2022/04
Summary	Human induced pluripotent stem cells (hiPSCs) are a promising cell source for elucidating disease pathology and therapy. The mass supply of hiPSC-derived cells is technically feasible. Carriers that can contain a large number of hiPSC-derived cells and evaluate their functions in vivo-like environments will become increasingly important for understanding disease pathogenesis or treating end-stage organ failure. hiPSC-derived hepatocyte-like cells (hiPSC-HLCs; 5 × 108) were seeded into decellularized organ-derived scaffolds under circumfusion culture. The scaffolds were implanted into immunodeficient microminiature pigs to examine their applicability in vivo. The seeded hiPSC-HLCs demonstrated increased albumin secretion and up-regulated cytochrome P450 activities compared with those in standard two-dimensional culture conditions. Moreover, they showed long-term survival accompanied by neovascularization in vivo. The decellularized organ-derived scaffold is a promising carrier for hiPSC-derived cells for ex vivo and in vivo use and is an essential platform for regenerative medicine and research.
DOI	10.3390/cells11081258
PMID	35455938