マツウラ　カツヒサ   MATSUURA Katsuhisa   松浦　勝久    所属   医学部 医学科    職種   教授・基幹分野長
論文種別	原著
言語種別	英語
査読の有無	査読なし
表題	Decellularized Organ-Derived Scaffold Is a Promising Carrier for Human Induced Pluripotent Stem Cells-Derived Hepatocytes.
掲載誌名	正式名：Cells 略　 称：Cells ISSNコード：20734409
掲載区分	国外
巻・号・頁	11(8),pp.1258
著者・共著者	KOJIMA Hideaki†, YAGI Hiroshi*, KUSHIGE Hiroko, TODA Yukiko, TAKAYAMA Kazuo, MASUDA Shinako, MORISAKU Toshinori, TSUCHIDA Tomonori, KURODA Kohei, HIRUKAWA Kazuya, INUI Jumpei, NISHI Kotaro, NAKANO Yutaka, TANAKA Masayuki, HORI Shutaro, HASEGAWA Yasushi, ABE Yuta, KIAGO Minoru, ADACHI Shungo, TOMI Masatoshi, MATSUURA Katsuhisa, MIZUGUCHI Hiroyuki, KITAGAWA Yuko
発行年月	2022/04
概要	Human induced pluripotent stem cells (hiPSCs) are a promising cell source for elucidating disease pathology and therapy. The mass supply of hiPSC-derived cells is technically feasible. Carriers that can contain a large number of hiPSC-derived cells and evaluate their functions in vivo-like environments will become increasingly important for understanding disease pathogenesis or treating end-stage organ failure. hiPSC-derived hepatocyte-like cells (hiPSC-HLCs; 5 × 108) were seeded into decellularized organ-derived scaffolds under circumfusion culture. The scaffolds were implanted into immunodeficient microminiature pigs to examine their applicability in vivo. The seeded hiPSC-HLCs demonstrated increased albumin secretion and up-regulated cytochrome P450 activities compared with those in standard two-dimensional culture conditions. Moreover, they showed long-term survival accompanied by neovascularization in vivo. The decellularized organ-derived scaffold is a promising carrier for hiPSC-derived cells for ex vivo and in vivo use and is an essential platform for regenerative medicine and research.
DOI	10.3390/cells11081258
PMID	35455938