Yoshina Sawako    Department   School of Medicine, School of Medicine    Position   Assistant Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	The small GTPase ARF-1.2 is a regulator of unicellular tube formation in Caenorhabditis elegans.
Journal	Formal name：The journal of physiological sciences : JPS Abbreviation：J Physiol Sci ISSN code：(1880-6562)1880-6546(Linking)
Domestic / Foregin	Domestic
Volume, Issue, Page	69(1),pp.47-56
Author and coauthor	Kage-Nakadai Eriko, Sun Simo, Iwata Satoru, Yoshina Sawako, Nishikawa Yoshikazu, Mitani Shohei
Publication date	2019/01
Summary	The membrane trafficking events that regulate unicellular tube formation and maintenance are not well understood. Here, using an RNAi screen, we identified the small GTPase ARF1 homolog ARF-1.2 as a regulator of excretory tube formation in Caenorhabditis elegans. RNAi-mediated knockdown and knockout of the arf-1.2 gene resulted in the formation of large intracellular vacuoles at the growth sites (varicosities) of the excretory canals. arf-1.2 mutant animals were sensitive to hyperosmotic conditions. arf-1.2 RNAi affected the localization of the anion transporter SULP-8, which is expressed in the basal plasma membrane of the excretory canals, but did not affect the expression of SULP-4, which is expressed in the apical membrane. The phenotype of arf-1.2 mutants was suppressed by mutation of the small Rho GTPase CDC-42, a regulator of apical/basal traffic balance. These results suggest that ARF-1.2 plays an essential role in basal membrane traffic to regulate the formation of the unicellular excretory tube.
DOI	10.1007/s12576-018-0617-5
PMID	29704149