ヨシナ　サワコ   Yoshina Sawako   吉名　佐和子    所属   医学部 医学科    職種   講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Distinct roles of the two VPS33 proteins in the endolysosomal system in Caenorhabditis elegans.
掲載誌名	正式名：Traffic (Copenhagen, Denmark) 略　 称：Traffic ISSNコード：(1600-0854)1398-9219(Linking)
掲載区分	国外
巻・号・頁	17(11),pp.1197-1213
著者・共著者	Gengyo-Ando Keiko, Kage-Nakadai Eriko, Yoshina Sawako, Otori Muneyoshi, Kagawa-Nagamura Yuko, Nakai Junichi, Mitani Shohei
発行年月	2016/11
概要	Sec1/Munc-18 (SM) family proteins are essential regulators in intracellular transport in eukaryotic cells. The SM protein Vps33 functions as a core subunit of two tethering complexes, class C core vacuole/endosome tethering (CORVET) and homotypic fusion and vacuole protein sorting (HOPS) in the endocytic pathway in yeast. Metazoan cells possess two Vps33 proteins, VPS33A and VPS33B, but their precise roles remain unknown. Here, we present a comparative analysis of Caenorhabditis elegans null mutants for these proteins. We found that the vps-33.1 (VPS33A) mutants exhibited severe defects in both endocytic function and endolysosomal biogenesis in scavenger cells. Furthermore, vps-33.1 mutations caused endocytosis defects in other tissues, and the loss of maternal and zygotic VPS-33.1 resulted in embryonic lethality. By contrast, vps-33.2 mutants were viable but sterile, with terminally arrested spermatocytes. The spermatogenesis phenotype suggests that VPS33.2 is involved in the formation of a sperm-specific organelle. The endocytosis defect in the vps-33.1 mutant was not restored by the expression of VPS-33.2, which indicates that these proteins have nonredundant functions. Together, our data suggest that VPS-33.1 shares most of the general functions of yeast Vps33 in terms of tethering complexes in the endolysosomal system, whereas VPS-33.2 has tissue/organelle specific functions in C. elegans.
DOI	10.1111/tra.12430
PMID	27558849