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KODAMA Takashi
Department School of Medicine, School of Medicine Position Assistant Professor |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | Tuba8 Drives Differentiation of Cortical Radial Glia into Apical Intermediate Progenitors by Tuning Modifications of Tubulin C Termini. |
| Journal | Formal name：Developmental cell Abbreviation：Dev Cell ISSN code：18781551/15345807 |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 52(4),pp.477-491.e8 |
| Author and coauthor | Ramos Susana I, Makeyev Eugene V, Salierno Marcelo, Kodama Takashi, Kawakami Yasuhiko, Sahara Setsuko |
| Publication date | 2020/02 |
| Summary | Most adult neurons and glia originate from radial glial progenitors (RGs), a type of stem cell typically extending from the apical to the basal side of the developing cortex. Precise regulation of the choice between RG self-renewal and differentiation is critical for normal development, but the mechanisms underlying this transition remain elusive. We show that the non-canonical tubulin Tuba8, transiently expressed in cortical progenitors, drives differentiation of RGs into apical intermediate progenitors, a more restricted progenitor type lacking attachment to the basal lamina. This effect depends on the unique C-terminal sequence of Tuba8 that antagonizes tubulin tyrosination and Δ2 cleavage, two post-translational modifications (PTMs) essential for RG fiber maintenance and the switch between direct and indirect neurogenesis and ultimately distinct neuronal lineage outcomes. Our work uncovers an instructive role of a developmentally regulated tubulin isotype in progenitor differentiation and provides new insights into biological functions of the cellular tubulin PTM "code." |
| DOI | 10.1016/j.devcel.2020.01.036 |
| PMID | 32097653 |