KODAMA Takashi    Department   School of Medicine, School of Medicine    Position   Assistant Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Neuronal classification and marker gene identification via single-cell expression profiling of brainstem vestibular neurons subserving cerebellar learning.
Journal	Formal name：The Journal of neuroscience : the official journal of the Society for Neuroscience Abbreviation：J Neurosci ISSN code：15292401/02706474
Domestic / Foregin	Foregin
Volume, Issue, Page	32(23),pp.7819-31
Author and coauthor	Kodama Takashi, Guerrero Shiloh, Shin Minyoung, Moghadam Seti, Faulstich Michael, du Lac Sascha
Authorship	Lead author
Publication date	2012/06
Summary	Identification of marker genes expressed in specific cell types is essential for the genetic dissection of neural circuits. Here we report a new strategy for classifying heterogeneous populations of neurons into functionally distinct types and for identifying associated marker genes. Quantitative single-cell expression profiling of genes related to neurotransmitters and ion channels enables functional classification of neurons; transcript profiles for marker gene candidates identify molecular handles for manipulating each cell type. We apply this strategy to the mouse medial vestibular nucleus (MVN), which comprises several types of neurons subserving cerebellar-dependent learning in the vestibulo-ocular reflex. Ion channel gene expression differed both qualitatively and quantitatively across cell types and could distinguish subtle differences in intrinsic electrophysiology. Single-cell transcript profiling of MVN neurons established six functionally distinct cell types and associated marker genes. This strategy is applicable throughout the nervous system and could facilitate the use of molecular genetic tools to examine the behavioral roles of distinct neuronal populations.
DOI	10.1523/JNEUROSCI.0543-12.2012
PMID	22674258