キクチ　テツタロウ   KIKUCHI Tetsutaro   菊地　鉄太郎    所属   研究施設 研究施設    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Non-coating method for non-adherent cell culture using high molecular weight dextran sulfate and bovine serum albumin.
掲載誌名	正式名：Journal of bioscience and bioengineering 略　 称：J Biosci Bioeng ISSNコード：13474421/13474421
掲載区分	国外
巻・号・頁	132(5),pp.537-542
著者・共著者	KIKUCHI Tetsutaro†*, MATSUURA Katsuhisa, SHIMIZU Tatsuya
担当区分	筆頭著者,責任著者
発行年月	2021/09/10
概要	Non-adherent cell culture surface has been widely used for producing cell spheroids and cell aggregates. The purpose of this study was to formulate a new method for non-adherent cell culture without coating or surface-modification that has been needed. We found that high-molecular-weight dextran sulfate (DS) and bovine serum albumin (BSA) synergistically prevented cell adhesion in media supplemented with no or low serum. This method worked on tissue culture-treated polystyrene surfaces as well as on commercially available low-attachment- and untreated polystyrene surfaces. Further investigation revealed that BSA may mediate the adsorption of DS to the surface. In addition, as the adsorption of fluorescently labeled fibronectin was inhibited by BSA alone, it appears that protein adsorption and cell adhesion do not always correlate. Finally, we demonstrated the successful formation of HepG2 spheroids and cardiomyocyte aggregates using this method. In conclusion, cell adhesion can be effectively suppressed by simply adding DS and BSA to the culture medium without coating or surface modification, and it may be useful for generating cell spheroids and aggregates.
DOI	10.1016/j.jbiosc.2021.08.006
PMID	34518107