Department   Research Institutes and Facilities, Research Institutes and Facilities
   Position   Assistant Professor
Article types Original article
Language English
Peer review Peer reviewed
Title Enhanced mechanical properties and cell separation with thermal control of PIPAAm-brushed polymer-blend microfibers.
Journal Formal name:Journal of materials chemistry. B
Abbreviation:J Mater Chem B
ISSN code:20507518/2050750X
Domestic / ForeginForegin
Publisher Royal Society of Chemistry
Volume, Issue, Page 8(28),pp.6017-6026
Author and coauthor NAGASE Kenichi†*, SHUKUWA Risa, TAKAHASHI Hironobu, TAKEDA Naoya*, OKANO Teruo*
Publication date 2020/07
Summary We have developed thermoresponsive microfibers with improved mechanical properties and enhanced temperature modulated-cell separation. Microfiber substrates were electrospun using poly(4-vinylbenzyl chloride) (PVBC)-poly(n-butyl methacrylate) (PBMA) blend materials in different ratios. Although their diameters were similar to those of the PVBC homofibers, polymer-blend microfibers exhibited excellent mechanical properties including non-brittle softness, owing to PBMA with a low Tg. These polymer-blend microfibers enabled the preparation of thin, dense mats that were superior in the experimental handling of cell separation. Poly(N-isopropylacrylamide) (PIPAAm) brushes were grafted via surface-initiated atom transfer radical polymerization from the initiation sites of PVBC in the polymer-blend microfiber substrates. The microfiber in a 25 : 75 ratio of PVBC : PBMA had a reasonable amount of the initiation sites and superior mechanical properties. The PIPAAm-brushed microfibers of the 25 : 75 blend substrate were capable of temperature-modulation, both in terms of wettability and cell separation. Among the normal human dermal fibroblasts (NHDFs), human umbilical vein endothelial cells (HUVECs), and human skeletal muscle myoblasts (HSMMs), HUVEC cells showed significantly poor adhesion on fibers at 37 °C; they were separated from adhered NHDF and HSMM cells in the initial step. Reducing the temperature to 20 °C remarkably detached NHDF cells, allowing their separation from HSMM cells. Compared with the PIPAAm-brushed PVBC homopolymer microfibers, these cell-separating functions were enhanced in the thermoresponsive PBMA-rich polymer-blend microfibers, probably ascribed to the properties of PBMA and the moderate density of the PIPAAm-brush. Thus, the developed microfibers could be useful for temperature-modulated cell separation systems.
DOI 10.1039/d0tb00972e
PMID 32573640