Takamitsu Miyayama    Department   School of Medicine, School of Medicine    Position   Assistant Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Separation of metallothionein isoforms extracted from isoform-specific knockdown cells on two-dimensional micro high-performance liquid chromatography hyphenated with inductively coupled plasma-mass spectrometry
Journal	Formal name：Journal of Analytical Atomic Spectrometry Abbreviation：J Anal Atom Spectrom ISSN code：0267-9477
Volume, Issue, Page	22(2),pp.179-182
Author and coauthor	MIYAYAMA Takamitsu†, OGRA Yasumitsu*, SUZUKI Kazuo
Authorship	Lead author
Publication date	2007
Summary	A two-dimensional (2D) micro HPLC-inductively coupled plasma mass spectrometry (ICP-MS) system consisting of a gel filtration column and an anion-exchange column was constructed to separate two metallothionein (MT) isoforms. MT-I and MT-II genes were specifically knocked down with the RNA interference (RNAi) technique, and the effects of the knockdown were evaluated by the reverse transcription-polymerase chain reaction (RT-PCR) and the 2D micro HPLC-ICP-MS system. The minimum cell number required by our 2D micro HPLC-ICP-MS system was calculated to be 2.0 x 10(3), and the detection limit of MT protein was estimated to be 22.2 mg L-1.
DOI	Doi 10.1039/B613662c
Document No.	ISI:000243976900012