ミヤヤマ タカミツ   Takamitsu Miyayama
  宮山 貴光
   所属   医学部 医学科
   職種   助教
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Separation of metallothionein isoforms extracted from isoform-specific knockdown cells on two-dimensional micro high-performance liquid chromatography hyphenated with inductively coupled plasma-mass spectrometry
掲載誌名 正式名:Journal of Analytical Atomic Spectrometry
略  称:J Anal Atom Spectrom
ISSNコード:0267-9477
巻・号・頁 22(2),179-182頁
著者・共著者 MIYAYAMA Takamitsu†, OGRA Yasumitsu*, SUZUKI Kazuo
発行年月 2007
概要 A two-dimensional (2D) micro HPLC-inductively coupled plasma mass spectrometry (ICP-MS) system consisting of a gel filtration column and an anion-exchange column was constructed to separate two metallothionein (MT) isoforms. MT-I and MT-II genes were specifically knocked down with the RNA interference (RNAi) technique, and the effects of the knockdown were evaluated by the reverse transcription-polymerase chain reaction (RT-PCR) and the 2D micro HPLC-ICP-MS system. The minimum cell number required by our 2D micro HPLC-ICP-MS system was calculated to be 2.0 x 10(3), and the detection limit of MT protein was estimated to be 22.2 mg L-1.
DOI Doi 10.1039/B613662c
文献番号 ISI:000243976900012