ミヤヤマ タカミツ   Takamitsu Miyayama
  宮山 貴光
   所属   医学部 医学科
   職種   助教
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Copper accumulation and compartmentalization in mouse fibroblast lacking metallothionein and copper chaperone, Atox1
掲載誌名 正式名:Toxicology and Applied Pharmacology
略  称:Toxicol Appl Pharmacol
ISSNコード:10960333/0041008X
巻・号・頁 237(2),205-13頁
著者・共著者 MIYAYAMA Takamitsu†, SUZUKI Kazuo, OGRA Yasumitsu*
発行年月 2009/06
概要 Copper (Cu) is the active center of some enzymes because of its redox-active property, although that property could have harmful effects. Because of this, cells have strict regulation/detoxification systems for this metal. In this study, multi-disciplinary approaches, such as speciation and elemental imaging of Cu, were applied to reveal the detoxification mechanisms for Cu in cells bearing a defect in Cu-regulating genes. Although Cu concentration in metallothionein (MT)-knockout cells was increased by the knockdown of the Cu chaperone, Atox1, the concentrations of the Cu influx pump, Ctr1, and another Cu chaperone, Ccs, were paradoxically increased; namely, the cells responded to the Cu deficiency despite the fact that cellular Cu concentration was actually increased. Cu imaging showed that the elevated Cu was compartmentalized in cytoplasmic vesicles. Together, the results point to the novel roles of MT and cytoplasmic vesicles in the detoxification of Cu in mammalian cells.
DOI 10.1016/j.taap.2009.03.024S0041-008X(09)00140-9 [pii]
文献番号 19362104