スエヒロ ユウジ   Suehiro Yuji
  末廣 勇司
   所属   医学部 医学科
   職種   講師
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Efficient collection of a large number of mutations by mutagenesis of DNA damage response defective animals.
掲載誌名 正式名:Scientific reports
略  称:Sci Rep
ISSNコード:20452322/20452322
掲載区分国外
巻・号・頁 11(1),pp.7630
著者・共著者 Suehiro Yuji, Yoshina Sawako, Motohashi Tomoko, Iwata Satoru, Dejima Katsufumi, Mitani Shohei
担当区分 筆頭著者
発行年月 2021/04
概要 With the development of massive parallel sequencing technology, it has become easier to establish new model organisms that are ideally suited to the specific biological phenomena of interest. Considering the history of research using classical model organisms, we believe that the efficient construction and sharing of gene mutation libraries will facilitate the progress of studies using these new model organisms. Using C. elegans, we applied the TMP/UV mutagenesis method to animals lacking function in the DNA damage response genes atm-1 and xpc-1. This method produces genetic mutations three times more efficiently than mutagenesis of wild-type animals. Furthermore, we confirmed that the use of next-generation sequencing and the elimination of false positives through machine learning could automate the process of mutation identification with an accuracy of over 95%. Eventually, we sequenced the whole genomes of 488 strains and isolated 981 novel mutations generated by the present method; these strains have been made available to anyone who wants to use them. Since the targeted DNA damage response genes are well conserved and the mutagens used in this study are also effective in a variety of species, we believe that our method is generally applicable to a wide range of animal species.
DOI 10.1038/s41598-021-87226-7
PMID 33828169