Suehiro Yuji
   Department   School of Medicine, School of Medicine
   Position   Assistant Professor
Article types Original article
Language English
Peer review Peer reviewed
Title An Aneuploidy-Free and Structurally Defined Balancer Chromosome Toolkit for Caenorhabditis elegans.
Journal Formal name:Cell reports
Abbreviation:Cell Rep
ISSN code:(2211-1247)
Domestic / ForeginForegin
Volume, Issue, Page 22(1),232-241頁
Author and coauthor Dejima Katsufumi†, Hori Sayaka†, Iwata Satoru†, Suehiro Yuji, Yoshina Sawako, Motohashi Tomoko, Mitani Shohei*
Publication date 2018/01
Summary Balancer chromosomes are critical tools for genetic research. In C. elegans, reciprocal translocations that lead to aneuploidy have been widely used to maintain lethal and sterile mutations in stable stocks. Here, we generated a set of aneuploidy-free and structurally defined crossover suppressors that contain two overlapping inversions using the CRISPR/Cas9 system. The toolkit includes 13 crossover suppressors and covers approximately 63% of all C. elegans coding genes. Together with the classical intrachromosomal crossover suppressors, the system now covers 89% of the coding genes. We also labeled the created balancers with fluorescent and phenotypic markers. We show that the crossover suppressors are better for embryonic analysis compared with translocational balancers. Additionally, we demonstrate an efficient method to generate lethal alleles by targeting essential genes on a chromosome balanced with a crossover suppressor. The toolkit will allow more efficient experiments in which lethal and sterile mutants can be analyzed.
DOI 10.1016/j.celrep.2017.12.024
PMID 29298424