モチヅキ　マキコ   望月　牧子    所属   医学部 医学科    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	The TIF1β-HP1 system maintains transcriptional integrity of hematopoietic stem cells.
掲載誌名	正式名：Stem cell reports 略　 称：Stem Cell Reports ISSNコード：22136711/22136711
掲載区分	国外
巻・号・頁	2(2),pp.145-52
著者・共著者	Miyagi Satoru, Koide Shuhei, Saraya Atsunori, Wendt George R, Oshima Motohiko, Konuma Takaaki, Yamazaki Satoshi, Mochizuki-Kashio Makiko, Nakajima-Takagi Yaeko, Wang Changshan, Chiba Tetsuhiro, Kitabayashi Issay, Nakauchi Hiromitsu, Iwama Atsushi
発行年月	2014/02
概要	TIF1β is a transcriptional corepressor that recruits repressive chromatin modifiers to target genes. Its biological function and physiological targets in somatic stem cells remain largely unknown. Here, we show that TIF1β is essential for the maintenance of hematopoietic stem cells (HSCs). Deletion of Tif1b in mice induced active cycling and apoptosis of HSCs and promoted egression of HSCs from the bone marrow, leading to rapid depletion of HSCs. Strikingly, Tif1b-deficient HSCs showed a strong trend of ectopic expression of nonhematopoietic genes. Levels of heterochromatin protein 1 (HP1α, β and γ) proteins, which form a complex with TIF1β, were significantly reduced in the absence of TIF1β and depletion of HP1 recapitulated a part of the phenotypes of Tif1b-deficient HSCs. These results demonstrate that the TIF1β-HP1 system functions as a critical repressive machinery that targets genes not normally activated in the hematopoietic compartment, thereby maintaining the transcriptional signature specific to HSCs.
DOI	10.1016/j.stemcr.2013.12.008
PMID	24527388