ヨシダ ケイタ   YOSHIDA Keita
  吉田 慶太
   所属   医学部 医学科
   職種   助教
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Germ cell mutations of the ascidian Ciona intestinalis with TALE nucleases.
掲載誌名 正式名:Genesis (New York, N.Y. : 2000)
略  称:Genesis
ISSNコード:1526-968X(Electronic)1526-954X(Linking)
掲載区分国外
巻・号・頁 52(5),431-9頁
著者・共著者 Yoshida Keita†, Treen Nicholas, Hozumi Akiko, Sakuma Tetsushi, Yamamoto Takashi, Sasakura Yasunori*
発行年月 2014/05
概要 Targeted mutagenesis of genes-of-interest, or gene-knockout, is a powerful method to address the functions of genes. Engineered nucleases have enabled this approach in various organisms because of their ease of use. The ascidian Ciona intestinalis is an excellent organism to analyze gene functions by means of genetic technologies. In our previous study, we reported mutagenesis of Ciona somatic cells with TALE nucleases (TALENs) by electroporating expression constructs. Inthis study, we report germ cell mutagenesis of Ciona by microinjecting mRNAs encoding TALENs. TALEN mRNAs introduced mutations to target genes in both somatic and germ cells. TALEN-mediated mutations in the germ cell genome were inherited by the next generation. We conclude that knockout lines of Ciona that have disrupted target genes can be established through TALEN-mediated germ cell mutagenesis.
DOI 10.1002/dvg.22770
文献番号 24619765