YOSHIDA Keita
   Department   School of Medicine, School of Medicine
   Position   Assistant Professor
Article types Original article
Language English
Peer review Peer reviewed
Title Chondroitin 6-O-sulfotransferases are required for morphogenesis of the notochord in the ascidian embryo.
Journal Formal name：Developmental dynamics : an official publication of the American Association of Anatomists
Abbreviation：Dev Dyn
ISSN code：1097-0177(Electronic)1058-8388(Linking)
Domestic / ForeginForegin
Volume, Issue, Page 243(12),pp.1637-45
Author and coauthor Nakamura Jun, Yoshida Keita, Sasakura Yasunori, Fujiwara Shigeki
Authorship 2nd author
Publication date 2014/12
Summary BACKGROUND:Chondroitin sulfate (CS) is a sulfated polysaccharide chain that binds to various core proteins to form proteoglycans. The amount and position of sulfate groups in CS are variable among different tissues, and are determined by specific sulfotransferases. Although the ascidians are the closest relatives of vertebrates, the functions of their sulfotransferases have not been studied.RESULTS:The genome of the ascidian Ciona intestinalis contains eight genes encoding proteins similar to chondroitin 6-O-sulfotransferases (C6STs), which appear to have independently diverged in the ascidian lineage during evolution. Among them, Ci-C6ST-like1 and Ci-C6ST-like7 were predominantly expressed in the developing notochord. In addition, they were weakly expressed in the neural tube. The disruption of either one of them affected the convergent extension movement of notochordal cells. Presumptive notochord cells coming from both sides of the embryo did not intercalate. The results suggest that both of them are necessary. In some cases, the anterior neural tube failed to close. Forced expression of Ci-C6ST-like1 or Ci-C6ST-liRESULTS:ke7 in the notochord restored the normal intercalation of notochordal cells, indicating that the effects of morpholino oligos are specific.CONCLUSIONS:Ci-C6ST-like1 and Ci-C6ST-like7 are required for the morphogenesis of the notochord in the ascidian embryo.
DOI 10.1002/dvdy.24213
Document No. 25298188