YOSHIDA Keita
Department School of Medicine, School of Medicine Position Assistant Professor |
|
Article types | Review article |
Language | English |
Peer review | Non peer reviewed |
Presence of invitation | Invited paper |
Title | Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease. |
Journal | Formal name:Methods in molecular biology (Clifton, N.J.) Abbreviation:Methods Mol Biol ISSN code:10643745/19406029 |
Domestic / Foregin | Foregin |
Volume, Issue, Page | 1630,pp.235-245 |
Author and coauthor | Sasakura Yasunori, Yoshida Keita, Treen Nicholas |
Authorship | 2nd author |
Publication date | 2017 |
Summary | The ascidian Ciona intestinalis is an important model animal for studying developmental mechanisms for constructing the chordate body. Although molecular and embryological techniques for manipulating Ciona genes were developed a long time ago, recent achievements of genome editing in this animal have innovated functional analyses of genes in Ciona. Particularly, knockout of genes in the G0 generation coupled with tissue-specific expression of TALENs enables us to rapidly address gene functions that were difficult using previous methods. |
DOI | 10.1007/978-1-4939-7128-2_19 |
Document No. | 28643263 |