YOSHIDA Keita
   Department   School of Medicine, School of Medicine
   Position   Assistant Professor
Article types Review article
Language English
Peer review Non peer reviewed
Presence of invitation Invited paper
Title Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease.
Journal Formal name:Methods in molecular biology (Clifton, N.J.)
Abbreviation:Methods Mol Biol
ISSN code:10643745/19406029
Domestic / ForeginForegin
Volume, Issue, Page 1630,pp.235-245
Author and coauthor Sasakura Yasunori, Yoshida Keita, Treen Nicholas
Authorship 2nd author
Publication date 2017
Summary The ascidian Ciona intestinalis is an important model animal for studying developmental mechanisms for constructing the chordate body. Although molecular and embryological techniques for manipulating Ciona genes were developed a long time ago, recent achievements of genome editing in this animal have innovated functional analyses of genes in Ciona. Particularly, knockout of genes in the G0 generation coupled with tissue-specific expression of TALENs enables us to rapidly address gene functions that were difficult using previous methods.
DOI 10.1007/978-1-4939-7128-2_19
Document No. 28643263